Enforced expression of GATA-3 during T cell development inhibits maturation of CD8 single-positive cells and induces thymic lymphoma in transgenic mice.

Lymphoma in Transgenic Mice Single-Positive Cells and Induces Thymic CD8 Cell Development Inhibits Maturation of Enforced Expression of GATA-3 During T

Enforced expression of GATA-3 in transgenic mice inhibits Th1 differentiation and induces the formation of a T1/ST2-expressing Th2-committed T cell compartment in vivo.

The transcription factor GATA-3 is essential for early T cell development and differentiation of naive CD4(+) T cells into Th2 effector cells. To study the function of GATA-3 during T cell-mediated immune responses in vivo, we investigated CD2-GATA3-transgenic mice in which GATA-3 expression is driven by the CD2 locus control region. Both in the CD4(+) and the CD8(+) T cell population the propo...

Enforced expression of Runx2 perturbs T cell development at a stage coincident with beta-selection.

The development of T cells in the thymus is regulated by a series of stage-specific transcription factors. Deregulated expression of these factors can lead to alterations in thymocyte development with the production of aberrant cell subsets and predispose to tumor formation. The three genes of the Runx family are multilineage regulators of differentiation that have been reported to be expressed...

BriefDefinitive Report A -y/b T Cell Receptor Heterodimer Induces the Expression of CD4 and CD8 in Thymocytes

CD4 and CD8 have been useful surface markers for a/R T cell maturation . In an a/(3 T cell receptor (TCR) transgenic SCID mice system, it has been shown that a/0 TCR alone is sufficient to induce CD4 and CD8 surface expression on thymic T cells. Although the late embryonic thymic y/b T cells are predominately single and double positive, it has not been clear if y/b TCR has a similar capacity. I...

Hyperresponse to T-cell receptor signaling and apoptosis of Id1 transgenic thymocytes.

The basic helix-loop-helix transcription factors, E2A and HEB, play important roles in T-cell development at multiple checkpoints. Expression of their inhibitor, Id1, abolishes the function of both transcription factors in a dose-dependent manner. The Id1 transgenic thymus is characterized by an accumulation of CD4- CD8- CD44+ CD25- thymocytes, a dramatic reduction of CD4+ CD8+ thymocytes, and ...